Desalación de péptidos
Purificación y desalinización de péptidos en enfoques proteómicos ascendentes con BioSPE® PurePep.
BioSPE® PurePep se utiliza para la desalación eficiente de péptidos después de la digestión enzimática, antes del análisis LC-MS/MS. Hasta el 97% de las proteínas se identifican después de desalar el digerido de HeLa en BioSPE® PurePep, con una buena recuperación de péptidos en todo el rango de polaridad, y una buena repetibilidad (RSD < 3%).
Para la desalación de los péptidos más hidrofílicos, recomendamos el uso de BioSPE® PurePep Broad, que puede retener eficazmente los péptidos polares pequeños. BioSPE® PurePep Broad también es estable en condiciones extremas de pH (pH 0-14) y puede secarse durante el proceso de SPE sin consecuencias en sus resultados.
Los kits BioSPE® PurePep y BioSPE™ PurePep Broad se componen de
- Columnas de desalación
- Adaptadores (para la centrifugación de puntas) y/o herramientas de recogida (tubos de centrifugación o placas de recogida). Cada kit contiene dos herramientas de recogida (una para los pasos de acondicionamiento/carga/lavado y otra para el paso de elución)
- Un protocolo detallado para una desalación rápida y sencilla
Para determinar el formato y la capacidad que mejor se adaptan a sus necesidades, consulte las tablas de la pestaña «Formatos y capacidades».
- Sin limitaciones de almacenamiento (se almacena en seco a temperatura ambiente)
- Diferentes formatos SPE disponibles para adaptarse al volumen de muestra y al rendimiento (Tips, columnas Spin, placas de 96 & 384 pocillos y cartuchos)
- Dos capacidades disponibles para cada formato en función de la cantidad de péptidos
Detalles sobre los volúmenes de carga y elución de nuestros diferentes productos BioSPE® PurePep para encontrar el mejor producto en función de su volumen de muestra.
Tabla de información sobre las capacidades (cantidad máxima de péptidos/proteínas que se pueden retener) de nuestros diferentes productos BioSPE® PurePep :
- Para la capacidad estándar, las referencias de los productos contienen la letra S (por ejemplo, Tips-PurePep.S.200.96).
- Para la capacidad alta, las referencias de los productos contienen la letra H (por ejemplo, Tips-PurePep.H.200.96)
Literatura
Hijazi, H. et al. (2025) “Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms”, Journal of Proteome Research.
En su última publicación, Hassan M. Hijazi et al. investigan la identificación de las variantes de histonas H3mm7 y H3mm13 específicas de ratón a partir de extractos de cerebro y testículo mediante LC-MS/MS.
Para permitir la detección precisa de modificaciones postraduccionales como la acetilación y la lactilación, se llevó a cabo la purificación de péptidos con Affinisep BioSPE® PurePep, que demostró ser muy eficaz para eliminar sustancias interferentes y preservar los péptidos modificados.
Este enfoque mejoró la calidad espectral MS/MS, en particular para iones críticos como b1 y CycIm, y ayudó a abordar los retos relacionados con las variantes isobáricas causadas por modificaciones no controladas como la propionilación.
Producto utilizado : BioSPE® PurePep Tips 200µl (KT-Adapt-Tips-PurePep.H.200.96)
Notas de aplicación
- Proteómica
Estimation of the working range on BioSPE™PurePep SPE Tips – Standard capacity
Nuevo !
Peptide desalting with BioSPE™ PurePep
Fraccionamiento de péptidos
Mejora de la identificación de proteínas por LC-MS/MS en muestras biológicas complejas como el plasma mediante el fraccionamiento de péptidos a pH básico.
Eliminación de perlas magnéticas
Eliminación de las perlas magnéticas de las muestras después de la preparación de muestras en una sola olla y con fase sólida (SP3).
Purificación de proteínas intactas
Purificación de proteínas intactas en enfoques proteómicos top-down.
Enriquecimiento de glicopéptidos
Purificación y enriquecimiento de glicanos y glicopéptidos.
LISTA DE PRODUCTOS
| Product Image | Nombre del producto | Product Description | Precio del producto | Acciones del carrito | |
|---|---|---|---|---|---|
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BioSPE™ PurePep – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep.S.1 |
96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep.H.1 |
96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep-Broad.H.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep-Broad.S.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep.H.1 |
96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep.S.1 |
96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep-Broad.H.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep-Broad.S.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.10.96 |
Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.10.96 |
Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters
Ref. : KT-Adapt-Tips-PurePep.SC.10.96 |
Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.1000.96 |
Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.1000.96 |
Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.200.96 |
Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk | Spin adapters - 96/pk | 2mL collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.200.96 |
Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Cartridges 3mL
Ref. : CAR3-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk |
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BioSPE™ PurePep – Spin
Ref. : Spin-PurePep.S.50 |
Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Cartridges 3mL
Ref. : CAR3-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 6mL
Ref. : CAR6-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 6mL
Ref. : CAR6-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk |
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|
BioSPE™ PurePep Broad – Spin
Ref. : Spin-PurePep-Broad.S.50 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk |
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|
BioSPE™ PurePep Broad – Spin
Ref. : Spin-PurePep-Broad.H.50 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Spin 15mL
Ref. : Spin15-PurePep-Broad.S.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 15mL
Ref. : Spin15-PurePep-Broad.H.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 50mL
Ref. : Spin50-PurePep-Broad.H.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 50mL
Ref. : Spin50-PurePep-Broad.S.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – µSpin
Ref. : µSpin-PurePep-Broad.S.50 |
µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – µSpin
Ref. : µSpin-PurePep-Broad.H.50 |
µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – µSpin
Ref. : µSpin-PurePep.S.50 |
µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – Cartridges 1mL
Ref. : CAR1-PurePep.S.50 |
SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 1mL
Ref. : CAR1-PurePep.H.50 |
SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 3mL
Ref. : CAR3-PurePep.S.50 |
SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 3mL
Ref. : CAR3-PurePep.H.50 |
SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 6mL
Ref. : CAR6-PurePep.S.50 |
SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 6mL
Ref. : CAR6-PurePep.H.50 |
SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk |
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BioSPE™ PurePep – Spin
Ref. : Spin-PurePep.H.50 |
Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – Spin 15mL
Ref. : Spin15-PurePep.H.25 |
Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 15mL
Ref. : Spin15-PurePep.S.25 |
Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 50mL
Ref. : Spin50-PurePep.H.25 |
Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 50mL
Ref. : Spin50-PurePep.S.25 |
Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk |
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|
BioSPE™ PurePep – µSpin
Ref. : µSpin-PurePep.H.50 |
µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk |
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|
BioSPE™ PurePep Broad – Cartridges 1mL
Ref. : CAR1-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 1mL
Ref. : CAR1-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk |
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